Distinct tubulin dynamics in cancer cells explored using a highly tubulin-specific fluorescent probe

Chem Commun (Camb). 2015 Sep 7;51(69):13400-3. doi: 10.1039/c5cc04927j. Epub 2015 Jul 27.

Abstract

A highly specific fluorescent probe (OC9) was discovered exhibiting tubulin-specific affinity fluorescence, which allowed selective labeling of cellular tubulin in microtubules. Moreover, distinct tubulin dynamics in various cellular bio-settings such as drug resistant or epithelial-mesenchymal transition (EMT) cancer cells were directly observed for the first time via OC9 staining.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds / chemistry*
  • Cell Line, Tumor
  • Chalcones / chemistry*
  • Drug Resistance, Neoplasm
  • Epithelial-Mesenchymal Transition
  • Fluorescent Dyes / chemistry*
  • Humans
  • MCF-7 Cells
  • Microscopy, Fluorescence
  • Microtubules / chemistry
  • Microtubules / metabolism
  • Tubulin / chemistry*
  • Tubulin / metabolism

Substances

  • 3-(4-(dimethylamino)phenyl)-1-(4'-fluoro(1,1'-biphenyl)-2-yl)prop-2-en-1-one
  • Aniline Compounds
  • Chalcones
  • Fluorescent Dyes
  • Tubulin