U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA

Richard G Wong; Katelynn Kazane; Dmitri A Maslov; Kestrel Rogers; Ruslan Aphasizhev; Larry Simpson

doi:10.1016/j.mito.2015.10.006

U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA

Mitochondrion. 2015 Nov:25:76-86. doi: 10.1016/j.mito.2015.10.006. Epub 2015 Oct 14.

Authors

Richard G Wong¹, Katelynn Kazane², Dmitri A Maslov³, Kestrel Rogers⁴, Ruslan Aphasizhev⁵, Larry Simpson⁶

Affiliations

¹ Department of Gerontology, Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089, United States; Department of Microbiology, Immunology and Molecular Genetics, Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States.
² Multispan Inc., Hayward, CA 94544, United States; Department of Microbiology, Immunology and Molecular Genetics, Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States.
³ Department of Biology, University of California - Riverside, Riverside, CA 92521, United States.
⁴ Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, United States.
⁵ Department of Molecular and Cell Biology, Boston University School of Dental Medicine, Boston, MA 02118, United States.
⁶ Department of Microbiology, Immunology and Molecular Genetics, Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States. Electronic address: larrys3255@gmail.com.

Abstract

We studied the intramitochondrial localization of several multiprotein complexes involved in U-insertion/deletion RNA editing in trypanosome mitochondria. The editing complexes are located in one or two antipodal nodes adjacent to the kinetoplast DNA (kDNA) disk, which are distinct from but associated with the minicircle catenation nodes. In some cases the proteins are in a bilateral sheet configuration. We also found that mitoribosomes have a nodal configuration. This type of organization is consistent with evidence for protein and RNA interactions of multiple editing complexes to form an ~40S editosome and also an interaction of editosomes with mitochondrial ribosomes.

Keywords: Antipodal node; Editing; Kinetoplast; Leishmania; RNA; Trypanosome.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

DNA, Kinetoplast / metabolism*
Leishmania / enzymology*
Leishmania / metabolism
Mitochondria / enzymology*
Mitochondria / metabolism
Mitochondrial Ribosomes / metabolism*
Multiprotein Complexes / metabolism*
RNA Editing*

Substances

DNA, Kinetoplast
Multiprotein Complexes

Abstract

Publication types

MeSH terms

Substances

Grants and funding