Objective: To investigate the effect of nano-realgar on proliferation and apoptosis of cervical cancer cells.
Methods: Different cervical cancer cell lines (Caski/HPV16+, adeno carcinoma; Hela/HPV18+, squmous carcinoma; C33A/HPV-, adeno carcinoma) were incubated with nano-realgar at different concentrations (5, 10, 20, 40 mg/L) for different times (24, 48, 72, 96 h). The morphology was observed under phase contrast microscope. The cell viability and apoptosis were examined by MTT and flow cytometry, respectively.
Results: The inhibitory effect of nano-realgar on the proliferation of cervical cancer cells was in a dose-dependent manner, with a range of inhibitory rate from 9.02% to 49.06%. Taking the group (20 mg/L) for an example, the inhibitory rates for Caski, Hela and C33A were 39.15%, 36.17% and 30.56%, respectively. The results of flow cytometry showed that the nano-realgar induced apoptosis in a concentration-dependent manner, with a range of apoptosis rate from 19.29% to 99.54%. Also taking the group (20 mg/L) for an example, the apoptosis rates for Caski, Hela and C33A were (60.43 ± 2.88)%, (41.95 ± 3.01)% and (43.49 ± 2.19)%, respectively. High concentration of nano-realgar (20 or 40 mg/L) could induce block of Hela and Caski at G2/M stage.
Conclusion: Nano-realgar can inhibit the proliferation of different cervical carcinoma cell lines and can induce the cell apoptosis. The inhibitory effect on cell proliferation is strongest for Caski, followed by Hela and C33A. It can also induce G2/M stage block on HPV positive cervical cancer cells at high enough concentration.
目的：探讨纳米雄黄对不同宫颈癌细胞株Hela(HPV18阳性，腺癌)，Caski(HPV16阳性，鳞癌)，C33A(HPV阴性，腺癌)增殖、凋亡的影响。方法：体外培养3种不同宫颈癌细胞，采用不同质量浓度(5，10，20，40 mg/L)纳米雄黄作用于不同宫颈癌细胞24，48，72，96 h，相差显微镜下观察不同质量浓度组与对照组细胞形态学变化；MTT法测定药物对细胞生长的抑制作用；流式细胞仪检测细胞凋亡及细胞周期。结果：纳米雄黄混悬液(5，10，20，40 mg/L)作用于Caski，Hela，C33A细胞24，48，72，96 h，均可抑制细胞增殖，且呈时间剂量依赖性，抑制率波动于9.02%~49.06%，以20 mg/L质量浓度组为例，抑制率Caski(39.15%)>Hela (36.17%)>C33A(30.56%)。随着质量浓度的增加，早期凋亡、中晚期凋亡细胞均增加，凋亡率(早期+中晚期凋亡率)波动于19.29%~99.54%，以20 mg/L质量浓度组为例，凋亡率Caski(60.43±2.88)%>Hela (41.95±3.01)%>C33A (43.49±2.19)%。细胞周期结果显示高质量浓度组(20，40 mg/L)对Hela，Caski细胞有G2/M期阻滞。结论：纳米雄黄混悬液可抑制不同宫颈癌细胞株的增殖，促进细胞凋亡，其对Caski细胞的作用最强，对Hela细胞次之，对C33A细胞相对最弱。对HPV阳性的细胞可产生G2/M期阻滞。.