Motivation: SpotCaliper is a novel wavelet-based image-analysis software providing a fast automatic detection scheme for circular patterns (spots), combined with the precise estimation of their size. It is implemented as an ImageJ plugin with a friendly user interface. The user is allowed to edit the results by modifying the measurements (in a semi-automated way), extract data for further analysis. The fine tuning of the detections includes the possibility of adjusting or removing the original detections, as well as adding further spots.
Results: The main advantage of the software is its ability to capture the size of spots in a fast and accurate way.
Availability and implementation: http://bigwww.epfl.ch/algorithms/spotcaliper/
Contact: zsuzsanna.puspoki@epfl.ch
Supplementary information: Supplementary data are available at Bioinformatics online.
© The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.