Competitive fluorescence assay for specific recognition of atrazine by magnetic molecularly imprinted polymer based on Fe3O4-chitosan

Guangyang Liu; Tengfei Li; Xin Yang; Yongxin She; Miao Wang; Jing Wang; Min Zhang; Shanshan Wang; Fen Jin; Maojun Jin; Hua Shao; Zejun Jiang; Hailong Yu

doi:10.1016/j.carbpol.2015.10.062

Competitive fluorescence assay for specific recognition of atrazine by magnetic molecularly imprinted polymer based on Fe3O4-chitosan

Carbohydr Polym. 2016 Feb 10:137:75-81. doi: 10.1016/j.carbpol.2015.10.062. Epub 2015 Oct 17.

Authors

Guangyang Liu¹, Tengfei Li², Xin Yang³, Yongxin She², Miao Wang², Jing Wang⁴, Min Zhang⁵, Shanshan Wang², Fen Jin², Maojun Jin², Hua Shao², Zejun Jiang², Hailong Yu²

Affiliations

¹ School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China; Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
² Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
³ School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China. Electronic address: yangxin@hit.edu.cn.
⁴ School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China; Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: w_jing2001@126.com.
⁵ School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China.

PMID: 26686107
DOI: 10.1016/j.carbpol.2015.10.062

Abstract

A novel fluorescence sensing strategy for determination of atrazine in tap water involving direct competition between atrazine and 5-(4,6-dichlorotriazinyl) aminofluorescein (5-DTAF), and which exploits magnetic molecularly imprinted polymer (MMIP), has been developed. The MMIP, based on Fe3O4-chitosan nanoparticles, was synthesized to recognize specific binding sites of atrazine. The recognition capability and selectivity of the MMIP for atrazine and other triazine herbicides was investigated. Under optimal conditions, the competitive reaction between 5-DTAF and atrazine was performed to permit quantitation. Fluorescence intensity changes at 515 nm was linearly related to the logarithm of the atrazine concentration for the range 2.32-185.4 μM. The detection limit for atrazine was 0.86μM (S/N=3) and recoveries were 77.6-115% in spiked tap water samples.

Keywords: Atrazine; Fe(3)O(4)-chitosan nanoparticles; Fluorescence assay; Magnetic molecularly imprinted polymers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Atrazine / chemistry*
Chitosan / chemistry*
Ferrosoferric Oxide / chemistry*
Fluorescence
Molecular Imprinting / methods*
Polymers / chemistry*

Substances

Polymers
Chitosan
Atrazine
Ferrosoferric Oxide