Retrieval of Mycobacterium tuberculosis cultures suspended in phosphate buffered saline

Int J Mycobacteriol. 2012 Sep;1(3):149-51. doi: 10.1016/j.ijmyco.2012.08.002. Epub 2012 Sep 1.

Abstract

One hundred and twenty-seven of 130 isolates of Mycobacterium tuberculosis, suspended in phosphate buffered saline (PBS) and stored at ambient conditions in the laboratory for 14days, and another 55 of 60 cultures, suspended as above, transported from reference laboratories within 7days, were successfully retrieved on LJ medium. Considering the maximum retrieval of M. tuberculosis, use of PBS can be explored further for transportation of M. tuberculosis cultures across laboratories.

Keywords: M. tuberculosis; MDR-TB; Phosphate buffer; Storage; Transportation.