Retrieval of Mycobacterium tuberculosis cultures suspended in phosphate buffered saline

V N Azger Dusthackeer; P Nagarajan; Dasarathi Das; Vanaja Kumar; N Selvakumar

doi:10.1016/j.ijmyco.2012.08.002

Retrieval of Mycobacterium tuberculosis cultures suspended in phosphate buffered saline

Int J Mycobacteriol. 2012 Sep;1(3):149-51. doi: 10.1016/j.ijmyco.2012.08.002. Epub 2012 Sep 1.

Authors

V N Azger Dusthackeer¹, P Nagarajan¹, Dasarathi Das², Vanaja Kumar¹, N Selvakumar³

Affiliations

¹ National Institute for Research in Tuberculosis (ICMR), Chennai, India.
² Regional Medical Research Centre (ICMR), Bhubaneshwar, India.
³ National Institute for Research in Tuberculosis (ICMR), Chennai, India. Electronic address: selvakumar.nagamiah@gmail.com.

PMID: 26787212
DOI: 10.1016/j.ijmyco.2012.08.002

Abstract

One hundred and twenty-seven of 130 isolates of Mycobacterium tuberculosis, suspended in phosphate buffered saline (PBS) and stored at ambient conditions in the laboratory for 14days, and another 55 of 60 cultures, suspended as above, transported from reference laboratories within 7days, were successfully retrieved on LJ medium. Considering the maximum retrieval of M. tuberculosis, use of PBS can be explored further for transportation of M. tuberculosis cultures across laboratories.

Keywords: M. tuberculosis; MDR-TB; Phosphate buffer; Storage; Transportation.