The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription

Mol Cell Biol. 1989 Dec;9(12):5602-9. doi: 10.1128/mcb.9.12.5602-5609.1989.

Abstract

To understand the function of SPT13, a gene encoding a trans-acting factor involved in regulation of Ty-mediated gene expression, we have cloned and sequenced the gene. Our analysis revealed that SPT13 is the same gene as GAL11, a gene in which mutations cause reduced expression of some GAL4-regulated genes. Further analysis of spt13/gal11 mutants suggested that the SPT13 gene also affects transcription of genes involved in mating-type specialization. We show here that SPT13 has both positive and negative regulatory roles in transcription.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Northern
  • Cloning, Molecular / methods
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal*
  • Genes, Regulator*
  • Genotype
  • Mating Factor
  • Mutation
  • Nucleic Acid Hybridization
  • Peptides / genetics
  • Plasmids
  • RNA, Fungal / genetics
  • RNA, Fungal / isolation & purification
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Trans-Activators / genetics*
  • Transcription, Genetic*

Substances

  • Peptides
  • RNA, Fungal
  • Trans-Activators
  • Mating Factor