Identification of synaptic acetylcholine receptor sites in retina with peroxidase-labeled alpha-bungarotoxin

Proc Natl Acad Sci U S A. 1977 Aug;74(8):3268-72. doi: 10.1073/pnas.74.8.3268.

Abstract

An alpha-bungarotoxin-horseradish peroxidase conjugate, which binds specificity to nicotinic acetylcholine receptors, was synthesized. This conjugate was bound by 5-7% of the synapses in the inner plexiform layer of the chicken retina. Bipolar ribbon synapses as well as amacrine synapses bound the conjugate.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetylcholine / metabolism*
  • Animals
  • Binding, Competitive
  • Bungarotoxins / metabolism*
  • Chickens
  • Diaphragm / metabolism
  • Female
  • Fishes
  • Horseradish Peroxidase
  • Mice
  • Microscopy, Electron
  • Molecular Weight
  • Motor Endplate / metabolism
  • Receptors, Cholinergic* / metabolism
  • Receptors, Nicotinic* / metabolism
  • Retina / metabolism*
  • Synapses / metabolism
  • Synapses / ultrastructure

Substances

  • Bungarotoxins
  • Receptors, Cholinergic
  • Receptors, Nicotinic
  • Horseradish Peroxidase
  • Acetylcholine