PLGA-based microparticles loaded with bacterial-synthesized prodigiosin for anticancer drug release: Effects of particle size on drug release kinetics and cell viability

J D Obayemi; Y Danyuo; S Dozie-Nwachukwu; O S Odusanya; N Anuku; K Malatesta; W Yu; K E Uhrich; W O Soboyejo

doi:10.1016/j.msec.2016.04.071

PLGA-based microparticles loaded with bacterial-synthesized prodigiosin for anticancer drug release: Effects of particle size on drug release kinetics and cell viability

Mater Sci Eng C Mater Biol Appl. 2016 Sep 1:66:51-65. doi: 10.1016/j.msec.2016.04.071. Epub 2016 Apr 21.

Authors

J D Obayemi¹, Y Danyuo², S Dozie-Nwachukwu³, O S Odusanya³, N Anuku⁴, K Malatesta⁵, W Yu⁶, K E Uhrich⁷, W O Soboyejo⁸

Affiliations

¹ Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, USA.
² Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Department of Materials Science and Engineering, Kwara State University, Malete, Ilorin, Kwara State, Nigeria.
³ Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria.
⁴ Department of Mechanical and Aerospace Engineering, Princeton University, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, USA; Department of Chemistry, Bronx Community College, New York, NY, USA.
⁵ Department of Mechanical and Aerospace Engineering, Princeton University, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, USA.
⁶ Department of Biomedical Engineering, Rutgers University, Piscataway, USA.
⁷ Department of Biomedical Engineering, Rutgers University, Piscataway, USA; Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, USA.
⁸ Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, USA. Electronic address: soboyejo@princeton.edu.

PMID: 27207038
DOI: 10.1016/j.msec.2016.04.071

Abstract

This paper presents the synthesis and physicochemical characterization of biodegradable poly (d,l-lactide-co-glycolide) (PLGA)-based microparticles that are loaded with bacterial-synthesized prodigiosin drug obtained from Serratia marcescens subsp. Marcescens bacteria for controlled anticancer drug delivery. The micron-sized particles were loaded with anticancer drugs [prodigiosin (PG) and paclitaxel (PTX) control] using a single-emulsion solvent evaporation technique. The encapsulation was done in the presence of PLGA (as a polymer matrix) and poly-(vinyl alcohol) (PVA) (as an emulsifier). The effects of processing conditions (on the particle size and morphology) are investigated along with the drug release kinetics and drug-loaded microparticle degradation kinetics. The localization and apoptosis induction by prodigiosin in breast cancer cells is also elucidated along with the reduction in cell viability due to prodigiosin release. The implication of this study is for the potential application of prodigiosin PLGA-loaded microparticles for controlled delivery of cancer drug and treatment to prevent the regrowth or locoregional recurrence, following surgical resection of triple negative breast tumor.

Keywords: Degradation; Drug release kinetics; Encapsulation efficiency; Microparticles; Paclitaxel; Poly (d,l-lactide-co-glycolide); Prodigiosin.

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology
Calorimetry, Differential Scanning
Cell Line, Tumor
Cell Survival / drug effects
Drug Carriers / chemistry
Drug Liberation
Humans
Lactic Acid / chemistry*
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Paclitaxel / chemistry
Paclitaxel / pharmacology
Particle Size
Polyglycolic Acid / chemistry*
Polylactic Acid-Polyglycolic Acid Copolymer
Prodigiosin / chemistry*
Prodigiosin / pharmacology
Serratia marcescens / chemistry
Serratia marcescens / metabolism

Substances

Antineoplastic Agents
Drug Carriers
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Lactic Acid
Prodigiosin
Paclitaxel