PLGA-based microparticles loaded with bacterial-synthesized prodigiosin for anticancer drug release: Effects of particle size on drug release kinetics and cell viability

Mater Sci Eng C Mater Biol Appl. 2016 Sep 1:66:51-65. doi: 10.1016/j.msec.2016.04.071. Epub 2016 Apr 21.

Abstract

This paper presents the synthesis and physicochemical characterization of biodegradable poly (d,l-lactide-co-glycolide) (PLGA)-based microparticles that are loaded with bacterial-synthesized prodigiosin drug obtained from Serratia marcescens subsp. Marcescens bacteria for controlled anticancer drug delivery. The micron-sized particles were loaded with anticancer drugs [prodigiosin (PG) and paclitaxel (PTX) control] using a single-emulsion solvent evaporation technique. The encapsulation was done in the presence of PLGA (as a polymer matrix) and poly-(vinyl alcohol) (PVA) (as an emulsifier). The effects of processing conditions (on the particle size and morphology) are investigated along with the drug release kinetics and drug-loaded microparticle degradation kinetics. The localization and apoptosis induction by prodigiosin in breast cancer cells is also elucidated along with the reduction in cell viability due to prodigiosin release. The implication of this study is for the potential application of prodigiosin PLGA-loaded microparticles for controlled delivery of cancer drug and treatment to prevent the regrowth or locoregional recurrence, following surgical resection of triple negative breast tumor.

Keywords: Degradation; Drug release kinetics; Encapsulation efficiency; Microparticles; Paclitaxel; Poly (d,l-lactide-co-glycolide); Prodigiosin.

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / pharmacology
  • Calorimetry, Differential Scanning
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Drug Carriers / chemistry
  • Drug Liberation
  • Humans
  • Lactic Acid / chemistry*
  • Microscopy, Atomic Force
  • Microscopy, Electron, Scanning
  • Microscopy, Fluorescence
  • Paclitaxel / chemistry
  • Paclitaxel / pharmacology
  • Particle Size
  • Polyglycolic Acid / chemistry*
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Prodigiosin / chemistry*
  • Prodigiosin / pharmacology
  • Serratia marcescens / chemistry
  • Serratia marcescens / metabolism

Substances

  • Antineoplastic Agents
  • Drug Carriers
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Prodigiosin
  • Paclitaxel