Objective: To study the expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells (PBMCs) in children with bronchial asthma, as well as their roles in the pathogenesis of asthma.
Methods: Thirty children with acute exacerbation of bronchial asthma were enrolled as the asthma group, and 20 healthy children were enrolled as the control group. RT-PCR and Western blot were used to measure the mRNA and protein expression levels of PI3K, NF-κB, and STAT1 in PBMCs. A spirometer was used to compare the pulmonary function between the two groups. The correlations between the mRNA expression of PI3K, NF-κB, and STAT1 and pulmonary function in children with bronchial asthma were analyzed.
Results: The asthma group had significantly higher mRNA and protein expression levels of PI3K, NF-κB, and STAT1 than the control group (P<0.05). Compared with the control group, the asthma group showed significant reductions in pulmonary function indices such as FEV1%, FEV1/FVC, and PEF% (P<0.05). In children with bronchial asthma, the mRNA expression levels of PI3K, NF-κB, and STAT1 were negatively correlated with FEV1%, FEV1/FVC, and PEF% (P<0.05).
Conclusions: The expression levels of PI3K, NF-κB, and STAT1 increase in children with asthma, and are negatively correlated with pulmonary function indices, suggesting that PI3K, NF-κB and STAT1 are involved in the development and progression of bronchial asthma in children.
目的: 检测PI3K、NF-κB和STAT1在支气管哮喘儿童外周血单个核细胞（PBMC）中的表达，探讨其在哮喘发病机制的临床意义。
方法: 选取30例急性发作期的支气管哮喘患儿作为哮喘组，随机选取同期健康儿童20例作为对照组。应用RT-PCR和Westernblot方法检测PBMC中PI3K、NF-κB和STAT1的mRNA和蛋白表达，肺功能仪检测两组肺功能，并分析支气管哮喘儿童PI3K、NF-κB和STAT1的mRNA水平与其肺功能的相关性。
结果: 哮喘组的PI3K、NF-κB和STAT1的mRNA和蛋白水平均高于对照组（P < 0.05）。与对照组相比，哮喘组FEV1%、FEV1/FVC、PEF%等肺功能指标均下降（P < 0.05）。哮喘儿童的PI3K、NF-κB和STAT1的mRNA表达水平与FEV1%、FEV1/FVC、PEF%均呈负相关（P < 0.05）。
结论: 哮喘患儿的PI3K、NF-κB和STAT1表达上调并与肺功能呈负相关，三者可能共同参与儿童支气管哮喘的发生、发展。