Assessing remyelination - metabolic labeling of myelin in an animal model of multiple sclerosis

Abstract

The lack of biomarkers is a major obstacle for investigating myelin repair. We used metabolic incorporation of the choline analog - propargyl-choline (P-Cho) to label and visualize newly synthesized myelin in the CNS of mice induced with experimental autoimmune encephalomyelitis (EAE). We further developed unbiased colocalization analysis to quantify P-Cho incorporation specifically into the myelin. Our findings indicate that P-Cho injection to mice recovering from EAE, either spontaneously or following glatiramer acetate treatment, results in significant elevation of its incorporation into the myelin, offering a novel strategy for assessing remyelination in animal models and the remyelination potential of candidate drugs.

Keywords: Experimental autoimmune encephalomyelitis (EAE); Glatiramer acetate (GA); In vivo metabolic labeling; Multiple sclerosis (MS); Quantitative colocalization analysis; Remyelination.