[The MicroRNA miR-205 inhibits epithelial-messenchymal transition in HK-2 cells by down-regulating ZEB1 and ZEB2 expressions]

Nan Fang Yi Ke Da Xue Xue Bao. 2016 Dec 20;36(12):1700-1705.
[Article in Chinese]

Abstract

Objective: To explore the role of miR-205 in regulating epithelial-messenchymal transition (EMT) in proximal tubular cell line HK-2 cells and the underlying mechanism.

Methods: HK-2 cells transfected with miR-205 mimics or a scrambled control sequence were examined for miR-205 expressions and mRNA levels of ZEB1, E-cadherin, and α-SMA using real-time qPCR; the protein levels of ZEB1, ZEB2, E-cadherin, and α-SMA were detected with Western blotting. Immunohistochemistry was performed to examine the ectopic expression of β-catenin and E-cadherin expression in the cells.

Results: The expression levels of ZEB1 and ZEB2 decreased significantly (P<0.01) while E-cadherin expression was up-regulated (P<0.01) in cells transfected with miR-205 mimics. Transfection with miR-205 mimics also markedly down-regulated the expression of α-SMA (P<0.01), a marker of mesenchymal cells that play an important role in EMT of HK-2 cells. The ectopic expression of β-catenin was inhibited by miR-205 mimics in HK-2 cells.

Conclusion: miR-205 inhibits EMT in HK-2 cells by down-regulating the expression levels of ZEB1 and ZEB2.

MeSH terms

  • Actins
  • Antigens, CD
  • Cadherins
  • Down-Regulation*
  • Epithelial-Mesenchymal Transition
  • Homeodomain Proteins*
  • Humans
  • Kidney Tubules, Proximal / metabolism*
  • MicroRNAs / physiology*
  • RNA, Messenger
  • Repressor Proteins
  • Transcription Factors
  • Up-Regulation
  • Zinc Finger E-box Binding Homeobox 2 / metabolism*
  • Zinc Finger E-box-Binding Homeobox 1 / metabolism*
  • beta Catenin

Substances

  • ACTA2 protein, human
  • Actins
  • Antigens, CD
  • CDH1 protein, human
  • CTNNB1 protein, human
  • Cadherins
  • Homeodomain Proteins
  • MIRN205 microRNA, human
  • MicroRNAs
  • RNA, Messenger
  • Repressor Proteins
  • Transcription Factors
  • ZEB1 protein, human
  • ZEB2 protein, human
  • Zinc Finger E-box Binding Homeobox 2
  • Zinc Finger E-box-Binding Homeobox 1
  • beta Catenin