Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid

Methods Mol Biol. 2017:1643:23-31. doi: 10.1007/978-1-4939-7180-0_3.

Abstract

Different protocols are required for the collection and isolation of antibodies from various body sites. For the sample collection factors to be considered include anatomic or physiological particularities. Secretory fluids such as saliva, gastrointestinal fluid, or breast milk may contain degrading enzymes that potentially affect the integrity of isolated antibodies. While the isolation of IgG from plasma is a common and often-described procedure, here we focus on methodological approaches to isolate antibodies immunoglobulin A (IgA) or IgM from plasma or secretory fluids. These protocols shall facilitate research on natural and induced antibodies.

Keywords: Antibody collection; Antibody isolation; Breast milk; Gastrointestinal fluid; Humoral immunity; IgA; IgM; Saliva; Secretory antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology*
  • Antibodies / isolation & purification*
  • Chromatography, Affinity
  • Gastric Juice*
  • Humans
  • Immunoglobulin A, Secretory / immunology
  • Immunoglobulin A, Secretory / isolation & purification
  • Immunoglobulin M / immunology
  • Immunoglobulin M / isolation & purification
  • Intestinal Secretions*
  • Milk, Human*
  • Plasma*
  • Saliva*

Substances

  • Antibodies
  • Immunoglobulin A, Secretory
  • Immunoglobulin M