Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors

Methods Mol Biol. 2017:1643:187-196. doi: 10.1007/978-1-4939-7180-0_15.

Abstract

Natural IgM antibodies have an innate ability to recognize many viruses and viral-based gene therapy vectors. Naive mice have natural IgM antibodies that bind to adenoviruses, and these antibodies can profoundly affect the biodistribution and efficiency of gene delivery by adenovirus type 5 vectors. Here, we present protocols for isolating IgM from mouse serum, for assaying the concentration and adenoviral reactivity of mouse IgM, and for evaluating how natural antibodies and complement can synergize to neutralize adenovirus vectors.

Keywords: Adenovirus type 5 vectors; Complement; Gene delivery; Mouse; Natural IgM antibodies; Virus.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviruses, Human / classification
  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / immunology*
  • Animals
  • Antibodies, Neutralizing / blood
  • Antibodies, Neutralizing / immunology
  • Antibodies, Viral / blood
  • Antibodies, Viral / immunology*
  • Complement System Proteins / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Gene Transfer Techniques
  • Genetic Therapy / adverse effects
  • Genetic Vectors / genetics
  • Genetic Vectors / immunology*
  • Humans
  • Immunoglobulin M / blood
  • Immunoglobulin M / immunology*
  • Mice

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Immunoglobulin M
  • Complement System Proteins