Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA

Biomol NMR Assign. 2017 Oct;11(2):265-268. doi: 10.1007/s12104-017-9760-9. Epub 2017 Aug 14.

Abstract

Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the 1H, 15N, and 13C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.

Keywords: Cancer development; Cell fate determination; Musashi; RBD; RNA-binding protein; RRM.

MeSH terms

  • Animals
  • Mice
  • Nerve Tissue Proteins / chemistry*
  • Nerve Tissue Proteins / metabolism*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Binding
  • Protein Domains
  • RNA / chemistry*
  • RNA / metabolism*
  • RNA-Binding Proteins / chemistry*
  • RNA-Binding Proteins / metabolism*

Substances

  • Msi1h protein, mouse
  • Nerve Tissue Proteins
  • RNA-Binding Proteins
  • RNA