Abstract
A simple method for obtaining prometaphase chromosomes from cultured first trimester cells involves the addition of BrdU and FdU 11 h before harvest and ethidium bromide 1.5 h before harvest. High resolution R-banding is obtained by acridine orange staining.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bromodeoxyuridine
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Chromosome Aberrations*
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Chromosome Banding / methods*
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Ethidium
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Female
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Floxuridine
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Humans
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Pregnancy
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Pregnancy Trimester, First
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Prenatal Diagnosis / methods*
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Trophoblasts / ultrastructure*
Substances
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Floxuridine
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Ethidium
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Bromodeoxyuridine