Dual reporter genetic mouse models of pancreatic cancer identify an epithelial-to-mesenchymal transition-independent metastasis program

EMBO Mol Med. 2018 Oct;10(10):e9085. doi: 10.15252/emmm.201809085.

Abstract

Epithelial-to-mesenchymal transition (EMT) is a recognized eukaryotic cell differentiation program that is also observed in association with invasive tumors. Partial EMT program in carcinomas imparts cancer cells with mesenchymal-like features and is proposed as essential for metastasis. Precise determination of the frequency of partial EMT program in cancer cells in tumors and its functional role in metastases needs unraveling. Here, we employed mesenchymal cell reporter mice driven by αSMA-Cre and Fsp1-Cre with genetically engineered mice that develop spontaneous pancreatic ductal adenocarcinoma (PDAC) to monitor partial EMT program. Both αSMA- and Fsp1-Cre-mediated partial EMT programs were observed in the primary tumors. The established metastases were primarily composed of cancer cells without evidence for a partial EMT program, as assessed by our fate mapping approach. In contrast, metastatic cancer cells exhibiting a partial EMT program were restricted to isolated single cancer cells or micrometastases (3-5 cancer cells). Collectively, our studies identify large metastatic nodules with preserved epithelial phenotype and potentially unravel a novel metastasis program in PDAC.

Keywords: dual‐recombinase system; metastasis; micrometastasis; pancreatic ductal adenocarcinoma; partial epithelial‐to‐mesenchymal transition.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Actins / metabolism
  • Animals
  • Disease Models, Animal
  • Epithelial-Mesenchymal Transition*
  • Genes, Reporter
  • Integrases / genetics
  • Integrases / metabolism
  • Mice
  • Neoplasm Metastasis / pathology
  • Neoplasm Metastasis / physiopathology
  • Pancreatic Neoplasms / pathology*
  • Pancreatic Neoplasms / physiopathology*
  • S100 Calcium-Binding Protein A4 / genetics
  • S100 Calcium-Binding Protein A4 / metabolism

Substances

  • Acta2 protein, mouse
  • Actins
  • S100 Calcium-Binding Protein A4
  • S100a4 protein, mouse
  • Cre recombinase
  • Integrases