Measuring In Vivo Tissue Metabolism Using 13C Glucose Infusions in Mice

Methods Mol Biol. 2019:1862:67-82. doi: 10.1007/978-1-4939-8769-6_5.

Abstract

Metabolic alterations are a hallmark of cancer. While determining metabolic changes in vitro has delivered valuable insight into the metabolism of cancer cells, it emerges that determining the in vivo metabolism adds an additional layer of information. Here, we therefore describe how to measure the in vivo metabolism of cancer tissue using 13C glucose infusions in mice.

Keywords: 13C glucose infusions; 13C tracer analysis; Cancer metabolism; In vivo metabolism; Metabolomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbon Isotopes / chemistry*
  • Gas Chromatography-Mass Spectrometry / instrumentation
  • Gas Chromatography-Mass Spectrometry / methods
  • Glucose / administration & dosage*
  • Glucose / chemistry
  • Glucose / metabolism
  • Metabolomics / instrumentation
  • Metabolomics / methods*
  • Mice
  • Mice, Inbred C57BL
  • Neoplasms / metabolism
  • Xenograft Model Antitumor Assays / instrumentation
  • Xenograft Model Antitumor Assays / methods

Substances

  • Carbon Isotopes
  • Carbon-13
  • Glucose