Guided Evolution of Recombinant Bombyx mori Acetylcholinesterase II by Homology Modeling to Change Pesticide Sensitivity

Jun Cai; Bingfeng Wang; Jiadong Li; Zijian Chen; Meifang Rao; Serge Muyldermans; Xiude Hua; Xi Xie; Hong Wang; Jinyi Yang; Zhenlin Xu; Yudong Shen; Yuanming Sun

doi:10.3390/ijms19113366

Guided Evolution of Recombinant Bombyx mori Acetylcholinesterase II by Homology Modeling to Change Pesticide Sensitivity

Int J Mol Sci. 2018 Oct 27;19(11):3366. doi: 10.3390/ijms19113366.

Authors

Jun Cai^{1

2}, Bingfeng Wang^{3

4}, Jiadong Li^{5

6}, Zijian Chen^{7

8}, Meifang Rao^{9

10}, Serge Muyldermans¹¹, Xiude Hua¹², Xi Xie^{13

14}, Hong Wang^{15

16}, Jinyi Yang^{17

18}, Zhenlin Xu^{19

20}, Yudong Shen^{21

22}, Yuanming Sun^{23

24}

Affiliations

¹ College of Food Science, South China Agricultural University, Guangzhou 510642, China. corrinej@stu.scau.edu.cn.
² Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. corrinej@stu.scau.edu.cn.
³ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. wbfeng@scau.edu.cn.
⁴ College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China. wbfeng@scau.edu.cn.
⁵ College of Food Science, South China Agricultural University, Guangzhou 510642, China. ljdong135@163.com.
⁶ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. ljdong135@163.com.
⁷ College of Food Science, South China Agricultural University, Guangzhou 510642, China. czj1q2w3e4r5t@stu.scau.edu.cn.
⁸ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. czj1q2w3e4r5t@stu.scau.edu.cn.
⁹ College of Food Science, South China Agricultural University, Guangzhou 510642, China. rmfrwl@163.com.
¹⁰ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. rmfrwl@163.com.
¹¹ Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinaan 2, 1050 Brussels, Belgium. serge.muyldermans@vub.be.
¹² College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. huaxiude@njau.edu.cn.
¹³ College of Food Science, South China Agricultural University, Guangzhou 510642, China. xix004@mail.usask.ca.
¹⁴ Department of Food and Bioproduct Sciences, University of Saskatchewan, S7N 5A8, Canada. xix004@mail.usask.ca.
¹⁵ College of Food Science, South China Agricultural University, Guangzhou 510642, China. gzwhongd@163.com.
¹⁶ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. gzwhongd@163.com.
¹⁷ College of Food Science, South China Agricultural University, Guangzhou 510642, China. yjy361@163.com.
¹⁸ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. yjy361@163.com.
¹⁹ College of Food Science, South China Agricultural University, Guangzhou 510642, China. jallent@163.com.
²⁰ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. jallent@163.com.
²¹ College of Food Science, South China Agricultural University, Guangzhou 510642, China. shenyudong@scau.edu.cn.
²² Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. shenyudong@scau.edu.cn.
²³ College of Food Science, South China Agricultural University, Guangzhou 510642, China. ymsun@scau.edu.cn.
²⁴ Guangdong Province Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. ymsun@scau.edu.cn.

Abstract

Keywords: docking; bottleneck; molecular dynamic simulation; side chain.

MeSH terms

Acetylcholinesterase / chemistry*
Acetylcholinesterase / genetics
Acetylcholinesterase / metabolism
Animals
Binding Sites
Bombyx / drug effects
Bombyx / enzymology*
Insect Proteins / chemistry*
Insect Proteins / genetics
Insect Proteins / metabolism
Insecticide Resistance*
Molecular Docking Simulation
Mutation, Missense
Pesticides / chemistry
Pesticides / pharmacology*
Protein Binding

Substances

Insect Proteins
Pesticides
Acetylcholinesterase

Abstract

MeSH terms

Substances

Grants and funding