Embryonic Immunostaining for the Tardigrade Hypsibius exemplaris

Frank W Smith; Willow N Gabriel

doi:10.1101/pdb.prot102343

Embryonic Immunostaining for the Tardigrade Hypsibius exemplaris

Cold Spring Harb Protoc. 2018 Nov 1;2018(11). doi: 10.1101/pdb.prot102343.

Authors

Frank W Smith¹, Willow N Gabriel²

Affiliations

¹ Biology Department, University of North Florida, Jacksonville, Florida 32224; frank.smith@unf.edu.
² Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.

PMID: 30385672
DOI: 10.1101/pdb.prot102343

Abstract

Immunostaining is a method used to visualize the localization of proteins in fixed tissue. Many antibodies are available that recognize specific proteins in a wide diversity of organisms, which makes this method ideal for investigating gene expression patterns in nonmodel animal systems. This protocol describes immunostaining for studies of embryogenesis in the tardigrade Hypsibius exemplaris.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Embryo, Nonmammalian / diagnostic imaging
Embryo, Nonmammalian / embryology
Embryo, Nonmammalian / metabolism*
Embryonic Development*
Immunohistochemistry / methods*
Microscopy, Fluorescence / methods
Tardigrada / embryology*