Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy

Elee Shimshoni; Irit Sagi

doi:10.1007/978-1-4939-9095-5_9

Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy

Methods Mol Biol. 2019:1944:129-133. doi: 10.1007/978-1-4939-9095-5_9.

Authors

Elee Shimshoni¹, Irit Sagi²

Affiliations

¹ Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel.
² Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel. irit.sagi@weizmann.ac.il.

PMID: 30840239
DOI: 10.1007/978-1-4939-9095-5_9

Abstract

Scanning electron microscopy is a useful tool for high-resolution morphological characterization of the extracellular matrix. In certain tissues and surfaces, imaging of the extracellular matrix requires cell removal before sample preparation. In this protocol, we will describe a method for preparing extracellular matrices derived from murine colon for imaging under a scanning electron microscope.

Keywords: Colon; Decellularization; Extracellular matrix; Scanning electron microscope; Tissue.

MeSH terms

Animals
Colon / metabolism*
Extracellular Matrix / metabolism*
Extracellular Matrix / ultrastructure
Mice
Microscopy, Electron, Scanning / methods*
Specimen Handling / methods*
Tissue Engineering / methods*
Tissue Scaffolds*