Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization

Mathilde H Lerche; Magnus Karlsson; Jan H Ardenkjær-Larsen; Pernille R Jensen

doi:10.1007/978-1-4939-9690-2_21

Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization

Methods Mol Biol. 2019:2037:385-393. doi: 10.1007/978-1-4939-9690-2_21.

Authors

Mathilde H Lerche¹, Magnus Karlsson¹, Jan H Ardenkjær-Larsen², Pernille R Jensen¹

Affiliations

¹ Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.
² Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark. jhar@elektro.dtu.dk.

PMID: 31463856
DOI: 10.1007/978-1-4939-9690-2_21

Abstract

Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized ¹³C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized ¹³C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.

Keywords: 13C-NMR; Glucose; Hyperpolarization; dDNP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon Isotopes / analysis*
Cell Polarity
Humans
Magnetic Resonance Spectroscopy / methods*
Metabolic Networks and Pathways*
Metabolomics / methods*

Substances

Carbon Isotopes