A novel radiolabeled graft polymer: Investigation of the radiopharmaceutical potential using Albino Wistar rats

Uğur Avcıbaşı; Buket Ateş; Perihan Ünak; Fikriye Gül Gümüşer; Süleyman Gülcemal; Kevser Kusat Ol; Sinan Akgöl; Volkan Tekin

doi:10.1016/j.apradiso.2019.108872

A novel radiolabeled graft polymer: Investigation of the radiopharmaceutical potential using Albino Wistar rats

Appl Radiat Isot. 2019 Dec:154:108872. doi: 10.1016/j.apradiso.2019.108872. Epub 2019 Aug 22.

Authors

Uğur Avcıbaşı¹, Buket Ateş², Perihan Ünak³, Fikriye Gül Gümüşer⁴, Süleyman Gülcemal⁵, Kevser Kusat Ol⁶, Sinan Akgöl⁷, Volkan Tekin³

Affiliations

¹ Department of Chemistry, Manisa Celal Bayar University, Faculty of Art and Science, 45140, Manisa, Turkey. Electronic address: ugur.avcibasi@cbu.edu.tr.
² Department of Chemistry, Manisa Celal Bayar University, Faculty of Art and Science, 45140, Manisa, Turkey.
³ Institute of Nuclear Sciences, Ege University, Department of Nuclear Applications, 35100, İzmir, Turkey.
⁴ Department of Nuclear Medicine, Manisa Celal Bayar University, School of Medicine, 45030, Manisa, Turkey.
⁵ Department of Chemistry, Ege University, Faculty of Science, 35100, Izmir, Turkey.
⁶ Turkish Health of Ministry, Turkish Medicines and Medical Devices Agency, Ankara, Turkey.
⁷ Department of Biochemistry, Faculty of Science, Ege University, 35100, İzmir, Turkey.

PMID: 31470192
DOI: 10.1016/j.apradiso.2019.108872

Abstract

Fe₃O₄ magnetic graft-Lys-poly(HEMA) was synthesized, labeled with ^99mTc for the first time and its radiopharmaceutical potential was investigated using animal models in this study. Quality control procedures were carried out using thin layer radiochromatography. The labeling yield of radiolabeled polymer was found to be about 100%. Then, stability and lipophilicity were determined. The lipophilicity of ^99mTc labeled Fe₃O₄ graft-Lys-poly(HEMA) was found to be 3.77. The serum stability experiments demonstrated that approximately 100% of radiolabeled polymer existed as an intact complex in the rat serum within 240 min. Biodistribution of radiolabeled magnetic graft-Lys-poly(HEMA) was performed on female Albino Wistar rats by scintigraphy and biodistribution studies. High uptake was seen in the stomach, the pancreas, brain, ovarian, intestines and the breast.

Keywords: Grafted polymer; HEMA; Nanoparticle; Tc-99m; l-lysine.

MeSH terms

Animals
Drug Stability
Female
Magnetite Nanoparticles / chemistry
Magnetite Nanoparticles / ultrastructure
Male
Microscopy, Electron, Scanning
Organotechnetium Compounds / chemical synthesis
Organotechnetium Compounds / chemistry*
Organotechnetium Compounds / pharmacokinetics
Polyhydroxyethyl Methacrylate / chemical synthesis
Polyhydroxyethyl Methacrylate / chemistry
Polyhydroxyethyl Methacrylate / pharmacokinetics
Polymers / chemical synthesis
Polymers / chemistry
Polymers / pharmacokinetics
Radiopharmaceuticals / chemical synthesis
Radiopharmaceuticals / chemistry*
Radiopharmaceuticals / pharmacokinetics
Rats
Rats, Wistar
Tissue Distribution

Substances

Magnetite Nanoparticles
Organotechnetium Compounds
Polymers
Radiopharmaceuticals
Polyhydroxyethyl Methacrylate