Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi003-A) from a Wilson's disease patient harboring a homozygous R778L mutation in ATP7B gene

Jiwei Zhang; Liting Wei; Dingbang Chen; Li Feng; Chao Wu; Rui Wang; Xunhua Li

doi:10.1016/j.scr.2019.101664

Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi003-A) from a Wilson's disease patient harboring a homozygous R778L mutation in ATP7B gene

Stem Cell Res. 2019 Dec:41:101664. doi: 10.1016/j.scr.2019.101664. Epub 2019 Nov 21.

Authors

Jiwei Zhang¹, Liting Wei², Dingbang Chen³, Li Feng³, Chao Wu³, Rui Wang⁴, Xunhua Li⁵

Affiliations

¹ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China. Electronic address: jiweizhang26@gmail.com.
² Department of Neurology, Luoyang Central Hospital affiliated to Zhengzhou University, Luoyang, Henan 467500, China.
³ Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
⁴ Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangzhou 510530, China.
⁵ Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China. Electronic address: lixunhua26@163.com.

PMID: 31783295
DOI: 10.1016/j.scr.2019.101664

Abstract

Human IPSC Line, ZZUNEUi003-A, was generated from a 32-year-old male patient with Wilson's Disease carrying a homozygous R778L mutation in ATP7B gene, using non-integrative reprogramming method. This cell line shows pluripotency both in vitro and vivo, and has a normal karyotype.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Base Sequence
Cell Culture Techniques / methods*
Cell Line / pathology*
Copper-Transporting ATPases / genetics*
Hepatolenticular Degeneration / genetics*
Hepatolenticular Degeneration / pathology*
Homozygote
Humans
Induced Pluripotent Stem Cells / pathology*
Male
Mutation / genetics*

Substances

ATP7B protein, human
Copper-Transporting ATPases