An adaptive excitation source for high-speed multiphoton microscopy

Nat Methods. 2020 Feb;17(2):163-166. doi: 10.1038/s41592-019-0663-9. Epub 2019 Dec 2.

Abstract

Optical imaging is important for understanding brain function. However, established methods with high spatiotemporal resolution are limited by the potential for laser damage to living tissues. We describe an adaptive femtosecond excitation source that only illuminates the region of interest, which leads to a 30-fold reduction in the power requirement for two- or three-photon imaging of brain activity in awake mice for improved high-speed longitudinal neuroimaging.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Action Potentials
  • Animals
  • Mice
  • Microscopy / methods*
  • Neuroimaging / methods
  • Neurons / physiology
  • Photons*