Effects of gelatin-based antifreeze peptides on cell viability and oxidant stress of Streptococcus thermophilus during cold stage

Xu Chen; Ling Li; Fujia Yang; Jinhong Wu; Shaoyun Wang

doi:10.1016/j.fct.2019.111056

Effects of gelatin-based antifreeze peptides on cell viability and oxidant stress of Streptococcus thermophilus during cold stage

Food Chem Toxicol. 2020 Feb:136:111056. doi: 10.1016/j.fct.2019.111056. Epub 2019 Dec 14.

Authors

Xu Chen¹, Ling Li², Fujia Yang³, Jinhong Wu⁴, Shaoyun Wang⁵

Affiliations

¹ College of Chemical Engineering, Fuzhou University, Fuzhou, Fujian, 350108, China; College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China.
² College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China; Yango University, Fuzhou, Fujian, 350015, China.
³ College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China.
⁴ Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
⁵ College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China. Electronic address: shywang@fzu.edu.cn.

PMID: 31846719
DOI: 10.1016/j.fct.2019.111056

Abstract

Cold stage adversely affects cell proliferation and cell viability of probiotics such as Streptococcus thermophilus in food industry, new type of cryoprotectants continues to be needed. Gelatin-based antifreeze peptide becomes a popular topic because of its cryoprotective effects on cold-stressed probiotics. In this study the effects of tilapia scales antifreeze peptides (TSAPP) on cell viability and oxidant stress of S. thermophilus during cold stage were investigated. The results showed that the percentage of viable cells was increased 10.85 folds compared with control groups. Addition of TSAPP activated the activities of ATPases, relieved the hyperpolarization of cell membrane potential and regulated the intracellular Ca²⁺ concentration. Furthermore, TSAPP significantly inhibited reactive oxygen species level and malonaldehyde content in cells. Under cryopreservation with TSAPP, cells of S. thermophilus maintained higher activities of antioxidant enzymes including catalase, peroxidase and total antioxidant capacity. These findings indicate that TSAPP likely offered its cellular protection by maintaining membrane integrity and alleviation of oxidative stress.

Keywords: Antifreeze peptides; Cryoprotective mechanism; Probiotics; Streptococcus thermophilus.

MeSH terms

Adenosine Triphosphatases / metabolism
Animal Scales / chemistry
Animals
Antifreeze Proteins / chemistry
Antifreeze Proteins / pharmacology*
Bacterial Proteins / metabolism
Cell Survival / drug effects*
Cold Temperature
Cryoprotective Agents / chemistry
Cryoprotective Agents / pharmacology*
Gelatin / chemistry
Gelatin / pharmacology
Membrane Potentials / drug effects
Molecular Weight
Oligopeptides / chemistry
Oligopeptides / pharmacology*
Oxidative Stress / drug effects*
Reactive Oxygen Species / metabolism
Streptococcus thermophilus / drug effects*
Tilapia

Substances

Antifreeze Proteins
Bacterial Proteins
Cryoprotective Agents
Oligopeptides
Reactive Oxygen Species
Gelatin
Adenosine Triphosphatases