Single pulsed electromagnetic field restores bone mass and microarchitecture in denervation/disuse osteopenic mice

Chih-Chun Lin; Yu-Ting Chang; Ru-Wei Lin; Chih-Wei Chang; Gwo-Jaw Wang; Kuo-An Lai

doi:10.1016/j.medengphy.2019.10.004

Single pulsed electromagnetic field restores bone mass and microarchitecture in denervation/disuse osteopenic mice

Med Eng Phys. 2020 Jun:80:52-59. doi: 10.1016/j.medengphy.2019.10.004. Epub 2020 May 4.

Authors

Chih-Chun Lin¹, Yu-Ting Chang², Ru-Wei Lin³, Chih-Wei Chang⁴, Gwo-Jaw Wang⁵, Kuo-An Lai⁶

Affiliations

¹ Medical Device Innovation Center, National Cheng Kung University, Tainan, Taiwan; Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Bone and Joint Research Center, National Cheng Kung University, Tainan, Taiwan. Electronic address: s974043@hotmail.com.
² Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan. Electronic address: qjemie@gmail.com.
³ Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Bone and Joint Research Center, National Cheng Kung University, Tainan, Taiwan; Institute of Food Safety Management, College of Agriculture, National Pingtung University of Science and Technology, Pingtung, Taiwan. Electronic address: rwlin@mail.npust.edu.tw.
⁴ Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Electronic address: u7901064@yahoo.com.tw.
⁵ Bone and Joint Research Center, National Cheng Kung University, Tainan, Taiwan; Department of Orthopedics, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan; Department of Orthopedic Surgery, University of Virginia, Charlottesville, VA, USA. Electronic address: gwojaw@cc.kmu.edu.tw.
⁶ Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Bone and Joint Research Center, National Cheng Kung University, Tainan, Taiwan. Electronic address: laikuoan@mail.ncku.edu.tw.

PMID: 32376078
DOI: 10.1016/j.medengphy.2019.10.004

Abstract

Pulsed electromagnetic fields (PEMFs) have been proposed to treat bone loss. However, time-consuming is the main complaint. A time-saving and effective treatment is of expectation. Previously, we showed a 3 min daily of single pulsed electromagnetic field (SPEMF) accelerated bone formation of long bone defect in mice. Here we compared the effect of SPEMF with PEMF for treating denervation/disuse osteopenic mice. Healthy mice were divided into 3 groups: intact mice (INT), INT + PEMF, and INT + SPEMF. Induced osteopenic mice were divided to osteopenia (IOP), IOP + PEMF, and IOP + SPEMF groups. The PEMF treated groups were subjected to daily 8 h PEMF(15 Hz, 18 G) exposure, while SPEMF treated groups were daily 3 min SPEMF(0.2 Hz, 1 T) exposure. BMD was evaluated every two weeks during the 12 weeks of treatment. Microarchitecture was evaluated on week 12. SPEMF significantly reversed bone loss in IOP mice as early as 6 weeks post-treatment, while PEMF reversed bone loss after 8 weeks. Bone volume was significantly increased in the IOP + PEMF and IOP + SPEMF group. Besides, bone volume and trabecular number of IOP + SPEMF mice were restored to the levels of INT mice in 12 weeks. Our finding suggests SPEMF increased BMD and restored microarchitecture of disuse osteopenic mice to healthy level.

Keywords: BMD (Bone mineral density); Bone microarchitecture; Disuse osteopenia; PEMF (pulsed electromagnetic field); SPEMF (single pulsed electromagnetic field).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Density*
Bone and Bones
Denervation
Electromagnetic Fields*
Mice
Osteogenesis