Generation of a human induced pluripotent stem cell line (SHUPLi001-A) from PBMCs of a healthy male donor

Yijun Cai; Junyi Lin; Kaijun Ma; Meng He; Beixu Li

doi:10.1016/j.scr.2020.101871

Generation of a human induced pluripotent stem cell line (SHUPLi001-A) from PBMCs of a healthy male donor

Stem Cell Res. 2020 Jun 10:47:101871. doi: 10.1016/j.scr.2020.101871. Online ahead of print.

Authors

Yijun Cai¹, Junyi Lin², Kaijun Ma³, Meng He⁴, Beixu Li⁵

Affiliations

¹ School of Policing Studies, Shanghai University of Political Science and Law, Shanghai 201701, PR China.
² Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China.
³ Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, PR China.
⁴ Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China. Electronic address: 042101055@fudan.edu.cn.
⁵ School of Policing Studies, Shanghai University of Political Science and Law, Shanghai 201701, PR China; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China. Electronic address: libeixu@fudan.edu.cn.

PMID: 32592952
DOI: 10.1016/j.scr.2020.101871

Abstract

Human induced pluripotent stem cells (iPSCs) are an ideal model for pathomechanism investigation and drug screening due to their differentiation potency. Here, we generated an iPSC line, SHUPLi001-A, from peripheral blood mononuclear cells (PBMCs) of a healthy 30-year-old male individual using non-integrating Sendai virus. Characterization of SHUPLi001-A was confirmed by the expression of typical pluripotency markers and by the teratoma formation assay to assess differentiation potency in vivo.