Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin

Hyosung Kim; Peng Jiang

doi:10.1016/j.xpro.2021.100443

Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin

STAR Protoc. 2021 Apr 7;2(2):100443. doi: 10.1016/j.xpro.2021.100443. eCollection 2021 Jun 18.

Authors

Hyosung Kim¹, Peng Jiang²

Affiliations

¹ Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37212, USA.
² Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.

Abstract

Current knowledge on brain oligodendrogenesis, composed of three sequential waves from different regions, is mainly obtained from rodent studies. Oligodendroglial development and myelination in the rodent brain may not fully mirror those processes in the human brain. Here, we provide a step-by-step protocol for generating fused forebrain organoids derived from human pluripotent stem cells. These fused organoids recapitulate human oligodendroglial developments, offering innovative insights into human myelination research in vitro. For complete details on the use and execution of this protocol, please refer to Cameron-Curry and Le Douarin (1995), Kessaris et al. (2006), and Kim et al. (2019).

Keywords: Cell Differentiation; Neuroscience; Organoids; Stem Cells.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Cell Culture Techniques / methods*
Cells, Cultured
Humans
Myelin Sheath / metabolism*
Oligodendroglia / metabolism*
Organoids* / cytology
Organoids* / metabolism
Pluripotent Stem Cells* / cytology
Pluripotent Stem Cells* / metabolism

Abstract

Publication types

MeSH terms

Grants and funding