Causal Effect of MMP-1 (Matrix Metalloproteinase-1), MMP-8, and MMP-12 Levels on Ischemic Stroke: A Mendelian Randomization Study

Stroke. 2021 Jul;52(7):e316-e320. doi: 10.1161/STROKEAHA.120.033041. Epub 2021 Apr 27.

Abstract

Background and purpose: MMP (matrix metalloproteinase) levels have been widely associated with ischemic stroke risk and poststroke outcome. However, their role as a risk factor or as a subeffect because of ischemia is uncertain.

Methods: We performed a literature search of genome-wide studies that evaluate serum/plasma levels of MMPs. We used a 2-sample Mendelian randomization approach to evaluate the causality of MMP levels on ischemic stroke risk or poststroke outcome, using 2 cohorts: MEGASTROKE (n=440 328) and GODs (n=1791).

Results: Genome-wide association studies of MMP-1, MMP-8, and MMP-12 plasma/serum levels were evaluated. A significant association, which was also robust in the sensitivity analysis, was found with all ischemic strokes: MMP-12 (odds ratio=0.90 [95% CI, 0.86–0.94]; q value=7.43×10−5), and with subtypes of stroke, large-artery atherosclerosis: MMP-1 (odds ratio=0.95 [95% CI, 0.92–0.98]; q value=0.01) and MMP-12 (odds ratio=0.71 [95% CI, 0.65–0.77]; q value=5.11×10−14); small-vessel occlusion: MMP-8 (odds ratio=1.24 [95% CI, 1.06–1.45]; q value=0.03). No associations were found in relation to stroke outcome.

Conclusions: Our study suggests a causal link between lower serum levels of MMP-12 and the risk of ischemic stroke, lower serum levels of MMP-1 and MMP-12 and the risk of large-artery stroke and higher serum levels of MMP-8 and the risk of lacunar stroke.

Keywords: atherosclerosis; ischemic stroke; metalloproteinase; modified Rankin Scale; risk factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / blood
  • Cohort Studies
  • Female
  • Genome-Wide Association Study / methods*
  • Humans
  • Ischemic Stroke / blood*
  • Ischemic Stroke / genetics
  • Male
  • Matrix Metalloproteinase 1 / blood*
  • Matrix Metalloproteinase 12 / blood*
  • Matrix Metalloproteinase 8 / blood*
  • Mendelian Randomization Analysis / methods*

Substances

  • Biomarkers
  • MMP8 protein, human
  • Matrix Metalloproteinase 8
  • MMP12 protein, human
  • Matrix Metalloproteinase 12
  • MMP1 protein, human
  • Matrix Metalloproteinase 1