Plasma Membrane Preparation from Lilium davidii and Oryza sativa Mature and Germinated Pollen

Abstract

Pollen germination is an excellent process to study cell polarity establishment. During this process, the tip-growing pollen tube will start elongating. The plasma membrane as the selectively permeable barrier that separates the inner and outer cell environment plays crucial roles in this process. This protocol described an efficient aqueous polymer two-phase system followed by alkaline solution washing to prepare Lilium davidii or Oryza sativa plasma membrane with high purity.

Keywords: Alkaline solution; Aqueous polymer two-phase system; Germinated pollen grains; Mature pollen grains; Plasma membrane.