Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase

Proc Natl Acad Sci U S A. 1987 Aug;84(16):5962-6. doi: 10.1073/pnas.84.16.5962.

Abstract

A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry
  • Calcium / metabolism*
  • Calmodulin / metabolism*
  • Cloning, Molecular*
  • DNA / analysis
  • Gene Expression Regulation
  • Isoenzymes / genetics*
  • Protein Kinases / genetics*
  • RNA, Messenger / metabolism
  • Rats

Substances

  • Calmodulin
  • Isoenzymes
  • RNA, Messenger
  • DNA
  • Protein Kinases
  • Calcium

Associated data

  • GENBANK/J02942