Transient focal ischemia induces a sustained downregulation of miR-7 leading to derepression of its target α-synuclein (α-Syn), which promotes neuronal death. We previously showed that treatment with miR-7 mimic prevents α-Syn induction and protects brain after stroke in rodents irrespective of age and sex. To further decipher the role of miR-7, we currently studied infarction and motor function in miR-7 double knockout mice (lack both miR-7a and miR-7b) subjected to focal ischemia. Adult miR-7-/- mice showed similar motor and cognitive functions to miR-7+/+ mice. However, when subjected to even a mild focal ischemia, the miR-7-/- mice showed exacerbated brain damage and worsened motor function compared with the miR-7+/+ mice. Replenishing miR-7 in miR-7-/- mice (IV injection of miR-7 mimic) restored miR-7 mediated neuroprotection and motor recovery, potentially by preventing α-Syn protein induction. Thus, we show that miR-7 is an essential miRNA in the brain that prevents α-Syn translation and the ensuing brain damage after stroke.
Keywords: Brain damage; Ischemia; Motor function; Noncoding RNA; Poststroke recovery.
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.