A novel signal enhancement strategy for the detection of DNA oxidative damage biomarker 8-OHdG based on the synergy between β-CD-CuNCs and multi-walled carbon nanotubes

Ruoting Lin; Shuang Zhou; Huanan Zhao; Huasong Lin; Lixing Wang; Weipeng Hu; Hongzhi Gao; Bin Qiu

A novel signal enhancement strategy for the detection of DNA oxidative damage biomarker 8-OHdG based on the synergy between β-CD-CuNCs and multi-walled carbon nanotubes

Am J Transl Res. 2022 Feb 15;14(2):740-751. eCollection 2022.

Authors

Ruoting Lin¹, Shuang Zhou², Huanan Zhao³, Huasong Lin¹, Lixing Wang², Weipeng Hu⁴, Hongzhi Gao^{2

4}, Bin Qiu³

Affiliations

¹ Department of Neurology, Second Affiliated Hospital of Fujian Medical University Quanzhou 362000, Fujian, China.
² Clinical Center of Molecular Diagnosis and Therapy, Second Affiliated Hospital of Fujian Medical University Quanzhou 362000, Fujian, China.
³ Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University Fuzhou 350108, Fujian, China.
⁴ Department of Neurosurgery, Second Affiliated Hospital of Fujian Medical University Quanzhou 362000, Fujian, China.

PMID: 35273682
PMCID: PMC8902525

Abstract

Objective: To propose a novel signal enhancement strategy based on the synergy between β-CD-CuNCs and multi-walled carbon nanotubes (MWCNTs) for the detection of DNA oxidative damage biomarker 8-Hydroxy-2'-deoxyguanosine (8-OHdG).

Methods: The sensor was constructed with the β-CD-CuNCs-MWCNTs-nafion film and successfully used for the quantitative detection of 8-OhdG in the presence of biomolecules such as ascorbic acid (AA) and uric acid (UA). To investigate the surface morphology of the modified electrode, Transmission Electron Microscopy (TEM), Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were performed on bare and modified electrodes.

Results: According to Differential Pulse Voltammetry (DPV) results, there was a linear relationship between peak current and concentration of 8-OhdG, ranging from 1.0×10^-7 to 1.0×10^-6 mol/L (R²=0.9926) and 1.0×10^-6 to 2.0×10^-5 mol/L (R²=0.9933). The detection limit (S/N=3) was 33 nmol/L.

Conclusions: The proposed sensor had been successfully applied to the determination of 8-OHdG in human urine samples with high recovery rates.

Keywords: 8-Hydroxy-2’-deoxyguanosine; Carboxyl-multi-walled carbon nanotubes; DNA oxidative damage; electrochemical biosensor; β-CD-CuNCs.