Cryo-EM Visualization of Neuronal Particles Inside Microtubules

Sylvie Gory-Fauré; Julie Delaroche; Camille Cuveillier; Christian Delphin; Isabelle Arnal

doi:10.1007/978-1-0716-1983-4_24

Cryo-EM Visualization of Neuronal Particles Inside Microtubules

Methods Mol Biol. 2022:2430:375-383. doi: 10.1007/978-1-0716-1983-4_24.

Authors

Sylvie Gory-Fauré¹, Julie Delaroche¹, Camille Cuveillier¹, Christian Delphin¹, Isabelle Arnal²

Affiliations

¹ Univ. Grenoble Alpes, Inserm U1216, CNRS, Grenoble Institut Neurosciences, Grenoble, France.
² Univ. Grenoble Alpes, Inserm U1216, CNRS, Grenoble Institut Neurosciences, Grenoble, France. isabelle.arnal@univ-grenoble-alpes.fr.

PMID: 35476345
DOI: 10.1007/978-1-0716-1983-4_24

Abstract

Neuronal microtubules have long been known to contain intraluminal particles, called MIPs (microtubule inner proteins), most likely involved in the extreme stability of microtubules in neurons. This chapter describes a cryo-electron microscopy-based assay to visualize microtubules containing neuronal MIPs. We present two protocols to prepare MIPs-containing microtubules, using either in vitro microtubule polymerization assays or extraction of microtubules from mouse hippocampal neurons in culture.

Keywords: Cryo-EM; MAP6; Microtubule inner proteins; Microtubules; Neurons.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cryoelectron Microscopy / methods
Hippocampus
Mice
Microtubules* / metabolism
Neurons*