DNP and ATP regulate the pulp breakdown development in Phomopsis longanae Chi-infected longan fruit through modulating the ROS metabolism

Yuzhao Lin; Hetong Lin; Lingzhen Zeng; Yi Zheng; Yazhen Chen; Zhongqi Fan; Yifen Lin

doi:10.1016/j.fochx.2022.100348

DNP and ATP regulate the pulp breakdown development in Phomopsis longanae Chi-infected longan fruit through modulating the ROS metabolism

Food Chem X. 2022 May 25:14:100348. doi: 10.1016/j.fochx.2022.100348. eCollection 2022 Jun 30.

Authors

Yuzhao Lin^{1

2}, Hetong Lin^{1

2}, Lingzhen Zeng^{1

2}, Yi Zheng^{1

2}, Yazhen Chen^{1

2}, Zhongqi Fan^{1

2}, Yifen Lin^{1

2}

Affiliations

¹ Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
² Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Province University, Fuzhou, Fujian 350002, China.

Abstract

Compared with the P. longanae-infected longan, the DNP-treated P. longanae-infected fruit represented a higher pulp breakdown index, a higher O₂ ^-. production rate, and a higher MDA content, but the lower activities of APX, SOD and CAT, the lower transcript levels of DlAPX6, DlSOD1, DlSOD2, DlSOD3 and DlCAT1, the lower values of AsA, GSH, flavonoid and total phenolics, a lower scavenging ability of DPPH radical, and a lower value of reducing power. Whereas, the ATP-treated P. longanae-infected samples showed the contrary results. The above findings indicated that the DNP-promoted the pulp breakdown in P. longanae-infected longan was because DNP weakened the capacity of scavenging ROS, raised the O₂ ^-. level, and accelerated the membrane lipids peroxidation. However, the ATP-suppressed the pulp breakdown in P. longanae-infected longan was because ATP improved the capacity of scavenging ROS, reduced the O₂ ^-. level, and reduced the membrane lipids peroxidation.

Keywords: 2,4-Dinitrophenol (DNP); APX, ascorbate peroxidase; ASA, acetylsalicylic acid; ASM, acibenzolar-S-methyl; ATP, adenosine triphosphate; Adenosine triphosphate (ATP); AsA, ascorbic acid; CAT, catalase; CE, catechin equivalent; DHEA, dehydroepiandrosterone; DNP, 2,4-dinitrophenol; DPPH, 1,1-diphenyl-2-picrylhydrazyl; DlSOD, DlCAT, and DlAPX denotes the gene of SOD, CAT, and APX in longan pulp, separately; GAE, gallic acid equivalent; GSH, glutathione; H2O2, hydrogen peroxide; Longan fruit; MDA, malondialdehyde; Membrane lipids peroxidation; NaClO, sodium hypochlorite; O2–., superoxide anion; P. longanae, Phomopsis longanae Chi; Phomopsis longanae Chi; Pulp breakdown; RH, relative humidity; RNA, ribose nucleic acid; ROS metabolism; ROS, reactive oxygen species; RSEs, ROS-scavenging enzymes; Reactive oxygen species (ROS); SDW, sterile distilled water; SOD, superoxide dismutase; cDNA, complementary deoxyribo nucleic acid; ·OH, hydroxyl radical.