Automated CUT & RUN Using the KingFisher Duo Prime

Abstract

Elucidating the biological function of histone methyltransferases requires knowledge of the genomic sites at which they act. CUT&RUN represents a valuable alternative to chromatin immunoprecipitation for the mapping of histone methylation patterns, generally producing results of equivalent quality while requiring less sequencing depth, less starting material and less effort. Automated CUT&RUN procedures have been developed to further facilitate chromatin profiling. Here we describe our automated CUT&RUN protocol using the Thermo Fisher KingFisher Duo Prime system.

Keywords: Automated CUT&RUN; Chromatin profiling; Easy CUT&RUN; Histone methylation; KingFisher.