Quercetin-Coating Promotes Osteogenic Differentiation, Osseointegration and Anti-Inflammatory Properties of Nano-Topographic Modificated 3D-Printed Ti6Al4V Implant

Nian Liu; Hui Wang; Zeyu Fu; Chuxi Zhang; Wenyu Hui; Jinyang Wu; Yong Zhang; Shilei Zhang

doi:10.3389/fbioe.2022.933135

Quercetin-Coating Promotes Osteogenic Differentiation, Osseointegration and Anti-Inflammatory Properties of Nano-Topographic Modificated 3D-Printed Ti6Al4V Implant

Front Bioeng Biotechnol. 2022 Jun 8:10:933135. doi: 10.3389/fbioe.2022.933135. eCollection 2022.

Authors

Nian Liu¹, Hui Wang¹, Zeyu Fu², Chuxi Zhang¹, Wenyu Hui¹, Jinyang Wu¹, Yong Zhang¹, Shilei Zhang¹

Affiliations

¹ Department of Oral and Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai, China.
² School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai, China.

Abstract

The capabilities of osseointegration and anti-inflammatory properties are of equal significance to the bio-inert titanium implant surface. Quercetin has proved its capacities of activating anti-inflammation through macrophage modulation and promoting osteogenic differentiation. Herein, we fabricated quercetin-coating on nano-topographic modificated 3D-printed Ti6Al4V implant surface. Subsequently the biological cells responses in vitro, anti-inflammatory and osseointegration performance in vivo were evaluated. In vitro studies indicated that quercetin-coating can enhance the adhesion and osteogenic differentiation of rBMSCs, while modulating the polarization of macrophages from M1 to M2 phase and improving the anti-inflammatory and vascular gene expression. Moreover, quercetin-loaded implants reduced the level of peri-implant inflammation and ameliorated new bone formation and rapid osseoinegration in vivo. Quercetin-coating might provide a feasible and favorable scheme for endowing 3D-printed titanium alloy implant surface with enhanced the rapid osseointegration and anti-inflammatory properties.

Keywords: anti-inflammation; macrophage polarization; micro-nano-topography; quercetin; rapid osseointegration; three-dimensional printing; titanium alloy.