Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2

Desarey Morales Vasquez; Kevin Chiem; Chengjin Ye; Luis Martinez-Sobrido

doi:10.1007/978-1-0716-2453-1_18

Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2

Methods Mol Biol. 2022:2524:235-248. doi: 10.1007/978-1-0716-2453-1_18.

Authors

Desarey Morales Vasquez¹, Kevin Chiem¹, Chengjin Ye¹, Luis Martinez-Sobrido^{2

3}

Affiliations

¹ Texas Biomedical Research Institute, San Antonio, TX, USA.
² Texas Biomedical Research Institute, San Antonio, TX, USA. lmartinez@txbiomed.org.
³ Department of Disease Prevention and Intervention, Texas Biomedical Research Institute, San Antonio, TX, USA. lmartinez@txbiomed.org.

Abstract

Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapter, we describe a detailed protocol on the generation of rSARS-CoV-2 expressing Venus, mCherry, and NLuc that represents a valid surrogate to track viral infections.

Keywords: Bioluminescence (BL); Coronavirus; Coronavirus disease 2019 (COVID-19); Fluorescence (FL); NanoLuc (NLuc); Recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Reporter genes; Reporter virus; Venus; mCherry.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

COVID-19*
Humans
Immunologic Tests
Respiratory System
SARS-CoV-2* / genetics

Abstract

Publication types

MeSH terms

Grants and funding