Reduction in the Migration Activity of Microglia Treated with Silica-Coated Magnetic Nanoparticles and their Recovery Using Citrate

Cells. 2022 Aug 3;11(15):2393. doi: 10.3390/cells11152393.

Abstract

Nanoparticles have garnered significant interest in neurological research in recent years owing to their efficient penetration of the blood-brain barrier (BBB). However, significant concerns are associated with their harmful effects, including those related to the immune response mediated by microglia, the resident immune cells in the brain, which are exposed to nanoparticles. We analysed the cytotoxic effects of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO2(RITC)] in a BV2 microglial cell line using systems toxicological analysis. We performed the invasion assay and the exocytosis assay and transcriptomics, proteomics, metabolomics, and integrated triple-omics analysis, generating a single network using a machine learning algorithm. The results highlight alteration in the mechanisms of the nanotoxic effects of nanoparticles using integrated omics analysis.

Keywords: exocytosis; integrated omics; metabolomics; microglia; migration; nanotoxicology; proteomics; transcriptomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Citrates
  • Citric Acid
  • Magnetite Nanoparticles*
  • Microglia
  • Silicon Dioxide* / pharmacology

Substances

  • Citrates
  • Magnetite Nanoparticles
  • Citric Acid
  • Silicon Dioxide

Grants and funding

This work was supported by grants from the National Research Foundation (NRF) and funded by the Ministry of Science and ICT (MSIT) in Korea (2020R1C1C1008366, 2020R1A4A4079722, and 2020M3E5D9080661). This work was supported by a KBSI grant (C270100) to Jin Young Kim.