Rat Pheochromocytoma PC12 Cells in Culture

Pin-Chun Chen; Chih-Tien Wang

doi:10.1007/978-1-0716-2671-9_1

Rat Pheochromocytoma PC12 Cells in Culture

Methods Mol Biol. 2023:2565:3-15. doi: 10.1007/978-1-0716-2671-9_1.

Authors

Pin-Chun Chen¹, Chih-Tien Wang²

Affiliations

¹ Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan.
² Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan. chihtienwang@ntu.edu.tw.

PMID: 36205883
DOI: 10.1007/978-1-0716-2671-9_1

Abstract

PC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.

Keywords: Dense-core vesicle; Fusion pore kinetics; Norepinephrine; PC12 cell culture; Proximity ligation assay with immunostaining; Rat pheochromocytoma; Regulated exocytosis; Single-cell biochemistry assay; Single-vesicle amperometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adrenal Gland Neoplasms*
Animals
Exocytosis
Kinetics
PC12 Cells
Pheochromocytoma*
Rats
Secretory Vesicles