Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Nonhomologous End Joining

Oliver H Krämer; Tamara Diehl; Wynand P Roos

doi:10.1007/978-1-0716-2788-4_19

Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Nonhomologous End Joining

Methods Mol Biol. 2023:2589:293-302. doi: 10.1007/978-1-0716-2788-4_19.

Authors

Oliver H Krämer¹, Tamara Diehl¹, Wynand P Roos²

Affiliations

¹ Institute of Toxicology, University Medical Center Mainz, Mainz, Germany.
² Institute of Toxicology, University Medical Center Mainz, Mainz, Germany. rooswy00@uni-mainz.de.

PMID: 36255632
DOI: 10.1007/978-1-0716-2788-4_19

Abstract

This book chapter describes a plasmid-based reporter method, first described by Bennardo et al. (2008) that we use in our laboratory for determining the activity of the repair of DNA double-strand breaks by nonhomologous end joining. This method can be used to measure the impact of epigenetic modifiers of the histone deacetylase family on this DNA repair pathway by flow cytometry.

Keywords: Class I histone deacetylases; Histone deacetylase inhibitors; Nonhomologous end joining.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA
DNA Breaks, Double-Stranded*
DNA End-Joining Repair
DNA Repair
Histone Deacetylase Inhibitors* / pharmacology
Histone Deacetylases

Substances

Histone Deacetylase Inhibitors
DNA
Histone Deacetylases