Parasite potpourri: Toxoplasma gondii lineage tracing in vivo

Trends Parasitol. 2022 Dec;38(12):1026-1027. doi: 10.1016/j.pt.2022.10.002. Epub 2022 Oct 25.

Abstract

Using a CRISPR/Cas9-based method, Wincott et al. generated a stable, complex Toxoplasma gondii population composed of 96 barcoded clonal lineages. By tracking the population structure in vivo, they determine that - contrary to expectations - the pathway to infecting the brain is widely permissive for T. gondii.

Keywords: Toxoplasma gondii; blood–brain barrier; dissemination; genetic bottleneck; molecular barcode.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural
  • Comment

MeSH terms

  • Animals
  • Parasites*
  • Toxoplasma* / genetics