Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories

Ruohao Tang; Xianzheng Yuan; Jianming Yang

doi:10.1016/j.biotechadv.2023.108183

Problems and corresponding strategies for converting CO₂ into value-added products in Cupriavidus necator H16 cell factories

Biotechnol Adv. 2023 Oct:67:108183. doi: 10.1016/j.biotechadv.2023.108183. Epub 2023 Jun 5.

Authors

Ruohao Tang¹, Xianzheng Yuan², Jianming Yang³

Affiliations

¹ Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, Shandong Key Lab of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, Shandong Province, People's Republic of China; Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, Shandong Province, People's Republic of China.
² Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, Shandong Province, People's Republic of China.
³ Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, Shandong Key Lab of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, Shandong Province, People's Republic of China. Electronic address: yjming888@126.com.

PMID: 37286176
DOI: 10.1016/j.biotechadv.2023.108183

Abstract

Elevated CO₂ emissions have substantially altered the worldwide climate, while the excessive reliance on fossil fuels has exacerbated the energy crisis. Therefore, the conversion of CO₂ into fuel, petroleum-based derivatives, drug precursors, and other value-added products is expected. Cupriavidus necator H16 is the model organism of the "Knallgas" bacterium and is considered to be a microbial cell factory as it can convert CO₂ into various value-added products. However, the development and application of C. necator H16 cell factories has several limitations, including low efficiency, high cost, and safety concerns arising from the autotrophic metabolic characteristics of the strains. In this review, we first considered the autotrophic metabolic characteristics of C. necator H16, and then categorized and summarized the resulting problems. We also provided a detailed discussion of some corresponding strategies concerning metabolic engineering, trophic models, and cultivation mode. Finally, we provided several suggestions for improving and combining them. This review might help in the research and application of the conversion of CO₂ into value-added products in C. necator H16 cell factories.

Keywords: CO(2); Cupriavidus necator H16; Formatotrophy; Metabolic engineering; Microbial electrosynthesis; Mixotrophy.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Carbon Dioxide* / metabolism
Cupriavidus necator* / genetics
Cupriavidus necator* / metabolism
Metabolic Engineering

Substances

Carbon Dioxide