Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells

Zoe Mattingly; Sundari Chetty

doi:10.1007/978-1-0716-3287-1_8

Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells

Methods Mol Biol. 2023:2683:89-101. doi: 10.1007/978-1-0716-3287-1_8.

Authors

Zoe Mattingly¹, Sundari Chetty^{2

3

4}

Affiliations

¹ Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA.
² Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA. schetty@mgh.harvard.edu.
³ Department of Psychiatry, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. schetty@mgh.harvard.edu.
⁴ Harvard Stem Cell Institute, Cambridge, MA, USA. schetty@mgh.harvard.edu.

PMID: 37300769
DOI: 10.1007/978-1-0716-3287-1_8

Abstract

Oligodendrocyte progenitor cells (OPCs) and mature oligodendrocytes (OLs) can be generated using human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs). By manipulating culture conditions, pluripotent cell types are serially guided through intermediary cell types, developing first into neural progenitor cells (NPCs) then OPCs before maturing as CNS-specific OLs. This procedure is conducted under adherent, feeder-free conditions to derive mature OLs in as few as 28 days.

Keywords: Differentiation; Human Embryonic Stem Cells (ESCs); Induced Pluripotent Stem Cells (iPSCs); Neural Progenitor Cells (NPCs); Neurons; Oligodendrocyte Progenitor Cells (OPCs); Oligodendrocytes (OLs).

MeSH terms

Cell Differentiation
Cells, Cultured
Embryonic Stem Cells
Humans
Induced Pluripotent Stem Cells*
Oligodendroglia