Coupled systems of pre-denitrification and partial nitritation/anammox improved functional microbial structure and nitrogen removal in treating swine manure digestate

Yunzhi Qian; Shilong He; Fuqiang Chen; Junhao Shen; Yan Guo; Yu Qin; Yu-You Li

doi:10.1016/j.biortech.2023.129494

Coupled systems of pre-denitrification and partial nitritation/anammox improved functional microbial structure and nitrogen removal in treating swine manure digestate

Bioresour Technol. 2023 Oct:386:129494. doi: 10.1016/j.biortech.2023.129494. Epub 2023 Jul 17.

Authors

Yunzhi Qian¹, Shilong He², Fuqiang Chen³, Junhao Shen³, Yan Guo⁴, Yu Qin⁵, Yu-You Li⁶

Affiliations

¹ School of Environment and Spatial Informatics, China University of Mining & Technology, Xuzhou 221116, China; Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
² School of Environment and Spatial Informatics, China University of Mining & Technology, Xuzhou 221116, China.
³ Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
⁴ Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.
⁵ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
⁶ Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan. Electronic address: gyokuyu.ri.a5@tohoku.ac.jp.

PMID: 37460018
DOI: 10.1016/j.biortech.2023.129494

Abstract

This study evaluated the functional activity and microbial structure of a pre-denitrification and single-stage partial nitritation/anammox process (DB-SNAP) coupled system for effectively treating swine manure digestate (SMD). At influent ammonium concentrations of (1000 to 1500) mg/L, the pre-denitrification reactor increased the nitrogen removal efficiency (NRE) by 5%, resulting in an average NRE of 96%. The DB-SNAP and nitrogen-limited strategy facilitated the rapid adoption of anammox bacteria (AnAOB) in the SMD, maintaining a high specific rate of 0.3gN/gVSS/d. A high secretion of tightly bound extracellular polymeric substances (76 mg/gVSS to 102 mg/gVSS) promoted micro-granule aggregation and stability. Moreover, Ca. Kuenenia, an AnAOB genus, was highly enriched from 21% to (27 to 30) %, whereas Nitrospira, a nitrite-oxidizing bacteria, was significantly suppressed to (0 to 0.05) %. These findings will provide valuable guidance in implementing the anammox process in swine wastewater treatment.

Keywords: EPS; Micro-granule; Microbial community; PN/A; Swine wastewater.

MeSH terms

Ammonium Compounds*
Anaerobic Ammonia Oxidation
Animals
Bacteria
Bioreactors / microbiology
Denitrification*
Manure
Nitrogen
Oxidation-Reduction
Sewage
Swine

Substances

Manure
Nitrogen
Ammonium Compounds
Sewage