Platinum nanoflowers stabilized with aloe polysaccharides for detection of organophosphorus pesticides in food

Han Zhao; Ruyu Li; Tingting Zhang; Lijie Zhou; Longgang Wang; Zengsheng Han; Sihang Liu; Jin Zhang

doi:10.1016/j.ijbiomac.2023.126552

Platinum nanoflowers stabilized with aloe polysaccharides for detection of organophosphorus pesticides in food

Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126552. doi: 10.1016/j.ijbiomac.2023.126552. Epub 2023 Sep 3.

Authors

Han Zhao¹, Ruyu Li², Tingting Zhang², Lijie Zhou², Longgang Wang³, Zengsheng Han², Sihang Liu⁴, Jin Zhang⁵

Affiliations

¹ Hebei Key Laboratory of Nano-biotechnology, Hebei Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China; State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.
² Hebei Key Laboratory of Nano-biotechnology, Hebei Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
³ Hebei Key Laboratory of Nano-biotechnology, Hebei Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China; State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China. Electronic address: lgwang@ysu.edu.cn.
⁴ State Key Laboratory of Advanced Optical Communication Systems and Networks, Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, UM-SJTU Joint Institute, Shanghai Jiao Tong University, Shanghai 200240, China.
⁵ Shanxi Datong University, College of Chemical and Environmental Engineering, Datong 037009, China.

PMID: 37660849
DOI: 10.1016/j.ijbiomac.2023.126552

Abstract

Organophosphorus pesticides can inhibit the activity of acetylcholinesterase and cause neurological diseases. Therefore, it is crucial to establish an efficient and sensitive platform for organophosphorus pesticide detection. In this work, we extracted aloe polysaccharide (AP) from aloe vera with the number average molecular weight of 27760 Da and investigated its reducing property. We prepared aloe polysaccharide stabilized platinum nanoflowers (AP-Pt_n NFs), their particle size ranges were 29.4-67.3 nm. Furthermore, AP-Pt_n NFs exhibited excellent oxidase-like activity and the catalytic kinetics followed the typical Michaelis-Menten equation. They showed strong affinity for 3,3',5,5'-tetramethylbenzidine substrates. More importantly, we developed a simple and effective strategy for the sensitive colorimetric detection of organophosphorus pesticides in food using biocompatible AP-Pt_n NFs. The detection range was 0.5 μg/L - 140 mg/L, which was wider than many previously reported nanozyme detection systems. This colorimetric biosensor had good selectivity and good promise for bioassay analysis.

Keywords: Aloe polysaccharide; Colorimetric assay; Organophosphorus pesticide.

MeSH terms

Acetylcholinesterase / chemistry
Aloe* / chemistry
Biosensing Techniques*
Colorimetry
Organophosphorus Compounds / analysis
Pesticides* / analysis
Platinum
Polysaccharides / chemistry

Substances

Pesticides
Organophosphorus Compounds
Acetylcholinesterase
Platinum
Polysaccharides