Comtrifosides A and B, two new triterpenoid saponins from the leaves of Combretum trifoliatum

Nat Prod Res. 2025 Jul;39(14):4104-4111. doi: 10.1080/14786419.2024.2332487. Epub 2024 Mar 22.

Abstract

The phytochemical composition of the Combretum trifoliatum leaves was studied for the first time. Two new triterpenoid saponins, named comtrifoside A (1) and comtrifoside B (2), together with two other saponins (3-4) were purified by variously chromatographic techniques. For the first time, compound 3 was informed from the Combretum genus, as well as all of the isolated compounds (1-4) were reported from C. trifoliatum. The chemical structures of them were clearly characterised using extensive UV-VIS, IR, HRMS-ESI, and NMR experimental data. The in vitro anti-inflammatory activities of 1 & 2 were examined against NO overproduction in LPS activation of RAW264.7.

Keywords: Combretum trifoliatum; anti-inflammatory activity; combretaceae; triterpenoid saponins.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents* / chemistry
  • Anti-Inflammatory Agents* / isolation & purification
  • Anti-Inflammatory Agents* / pharmacology
  • Combretum* / chemistry
  • Lipopolysaccharides / pharmacology
  • Mice
  • Molecular Structure
  • Nitric Oxide / biosynthesis
  • Plant Leaves* / chemistry
  • RAW 264.7 Cells
  • Saponins* / chemistry
  • Saponins* / isolation & purification
  • Saponins* / pharmacology
  • Triterpenes* / chemistry
  • Triterpenes* / isolation & purification
  • Triterpenes* / pharmacology

Substances

  • Saponins
  • Triterpenes
  • Anti-Inflammatory Agents
  • Nitric Oxide
  • Lipopolysaccharides