Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn7 transposition. Here, we optimized methods specific for applying Mobile-CRISPRi in multiple Vibrio species.
Keywords: CRISPR; CRISPRi; Vibrio; Vibrio campbellii; Vibrio cholerae; Vibrio fischeri; Vibrio parahaemolyticus; Vibrio vulnificus; regulation of gene expression.