Effect of Hafnia alvei on Morphophysiologic Parameters and Gut Microbiota of Mice with Inherited Type 2 Diabetes Mellitus

Bull Exp Biol Med. 2024 Jul;177(3):313-317. doi: 10.1007/s10517-024-06180-2. Epub 2024 Aug 10.

Abstract

Inbred mouse strains KK.Cg-a/a and KK.Cg-Ay/a known as genetic models of type 2 diabetes mellitus significantly surpassed the control strain C57BL/6J in the body weight, relative weight of extractable fat, and basal blood glucose levels. Real-timePCR of fecal samples from KK.Cg-a/a and KK.Cg-Ay/a mice revealed dysbiosis typical of type 2 diabetes mellitus in humans and animals. Long-term intragastric administration of a suspension of Hafnia alvei bacteria had no effect on the above morphometric and biochemical parameters. At the same time, recovery of the Bacteroides spp. population in KK.Cg-Ay/a mice and a decrease in the number of Bifidobacterium spp. in KK.Cg-a/a mice were observed. The possibility of therapeutic use of the probiotic based on H. alvei is discussed.

Keywords: Hafnia alvei; energy metabolism; inbred mouse strains; microbiota; type 2 diabetes mellitus.

MeSH terms

  • Animals
  • Bacteroides / genetics
  • Bifidobacterium / genetics
  • Blood Glucose / metabolism
  • Body Weight
  • Diabetes Mellitus, Type 2* / genetics
  • Diabetes Mellitus, Type 2* / microbiology
  • Diabetes Mellitus, Type 2* / pathology
  • Disease Models, Animal
  • Dysbiosis / microbiology
  • Feces* / microbiology
  • Gastrointestinal Microbiome* / genetics
  • Hafnia alvei* / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL*
  • Probiotics* / administration & dosage

Substances

  • Blood Glucose